目的 研究海绵共生真菌Beauveria felina SX-6-22的次级代谢产物。方法 采用硅胶、ODS、半制备HPLC等方法对菌株固体发酵物提取物分离纯化,运用NMR、MS等现代波谱学方法鉴定化合物的结构,通过X-ray单晶衍射技术确定了新化合物1的绝对构型。结果 分离得到9个次级代谢产物,包括4个新化合物beauverins A~D(1~4),以及已知化合物1,9-dimethyl-3,7-dibenzofurandiol(5),diorcinol(6),cordyol C(7),violaceolⅠ(8)和violaceolⅡ(9)。化合物5,7,8和9具有抗氧化活性,DPPH的IC50分别为(9.62±0.12),(11.11±0.02)和(8.44±0.08)μg·mL-1。结论 从海绵内生真菌SX-6-22中发现了4个新颖化合物,以及4个具有较强抗氧化活性的化合物。
Abstract
OBJECTIVE To study the secondary metabolites of sponge-associated fungus Beauveria feline SX-6-22.METHODS The compounds were isolated and purified by silica gel, ODS reversed-phase column, preparative HPLC techniques. And their structures were identified by the physicochemical properties and NMR, MS data analysis. X-ray crystal diffraction was used to confirm the absolute configuration of compound1.RESULTS Nine secondary metabolites were isolated from the extract of the solid fermentation culture of strain SX-6-22, including four new compounds beauverins A-D (1-4) and five known compounds 1, 9-dimethyl-3, 7-dibenzofurandiol (5), diorcinol(6), cordyol C(7), mixture of violaceol Ⅰ(8) and violaceol Ⅱ(9). Compounds 5, 7, 8 and 9 showed strong antioxidant activity with IC50 (9.62±0.12), (11.11±0.02), and (8.44±0.08) μg·mL-1 by DPPH assay.CONCLUSION Four new compounds and three antioxidant known compounds are isolated from sponge-associated fungus SX-6-22.
关键词
海绵共生真菌 /
Beauveria felina /
次级代谢产物 /
抗氧化
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Key words
sponge-associated fungi /
Beauveria felina /
secondary metabolites /
antioxidant
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中图分类号：
R284
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参考文献
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